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Thylakoids have a self-repairing ability to accumulate free magnesium against light stress, which is mediated by a compartmentalized intracellular free Mg(2+) accumulation through Mg-ATPase (Magnesium-adenosine triphosphatase). To understand a physiological function of Mg(2+) sequestered in thylakoids, a molecular genetic strategy was used to disrupt the function of genes for magnesium transport and sequestration. This strategy is based on a specific insertion of recombinant DNAs into thylakoid membrane using the transformation system based on the green fluorescent protein. This strategy was used to disrupt the function of a newly identified magnesium transporter gene, NTT, which is a member of the 2A proton-magnesium transporter family. It was found that the disruption of NTT gene resulted in the accumulation of more than one order of magnitude of magnesium in the mutant thylakoid. This analysis is the first to demonstrate a self-repair ability of thylakoids for magnesium accumulation. The thylakoids were extremely insoluble and trapped several types of enzymes by a high magnesium environment. The soluble form of enzymes is directly linked to a self-repair function of the thylakoids. The mutant
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Recipes (Semmelweiss, Adolph Maximilian Wermuth, ) Find great deals on eBay for ace bandura and primary colors. Shop with confidence. Ask HN: Raising Funding? – nikladd I just started a product that is aimed at helping people find and learn skills faster. The challenge is, it has zero traction and I'm not even sure what stage of development it is at. I will be building it out using Bootstrap and React. I have no prior experience with React, and I have only a vague idea of what Bootstrap is. In fact, my project has never had a codebase.I've done my time reading research, reading docs, and figuring out how to build the things I want to build. Now it is the time to actually build, but the problem is that most of my team skills are in the research phase (like analytics, etc.)Do you have any advice on what I need to do in order to make progress without the team?FYI, I'm not really asking how to find funding. My question is more of a "How do I best work with a new team?" question. ====== charlieegan3 You did say zero traction. I know some people who would have not quit their jobs to work on this. It seems like this product is exactly the sort of thing that companies with huge infrastructures want to build: very relevant, valuable, not using technology that’s in use/supported by their ecosystem, just a plain to build upon. You can’t afford to spend time learning new technologies? You’ve got to make more money. Try to find a customer who can give you feedback. Tell them what you need, offer to get it for them (via a partner you can bring in, or make your partner). Good luck. Best of luck. ~~~ nikladd Great thoughts. Thanks for the reply. I can’t afford to spend time learning new technologies, because my product is in its early stages, and my progress is unlikely to make me any more money in the next few years. I have a feeling that it might be easier to sell to a larger company than